Multiple Fermentation System

Tank system

Volume: total volume 10/20/30/40/50/100/200/300/500/1000L, maximum working volume 70%.

All stainless steel tank body has no dead corners in the tank, and the side of the tank body adopts a large-view glass to observe the liquid level in the tank.

Material: Tank: SUS316L; Jacket: SUS304.

Diameter to height ratio: 1.5:2.

Tank lid: With defoaming, inoculation port, multiple feeding ports, exhaust ports, etc...12V (Philips) safety sight light.

Tank bottom: With fermentation tank bottom valve and discharging valve transfer port (quick connection method), special design, no dead corners and liquid accumulation, steam sterilization.

Tank body: lLng sight glass, temperature electrode interface, pH electrode interface, DO electrode interface, sampling valve port.

Design pressure: Tank body 0.3Mpa.

Jacket: Design pressure 0.3Mpa.

Surface treatment: internal and external mirror polishing, the accuracy of the tank is less than 0.4 um to reduce the chance of contamination (Using electrolytic polishing), and the accuracy of the outer surface is less than 0.6 um.

Sterilization method: Online steam sterilization (Using automatic sterilization).

Intake system

Equipped with four-way air intake, including: rotameter, mass flow meter, 2-stage sterilization filter, check valve, etc., to ensure sterile air entering the tank.

The mass flowmeter uses the Swiss Fordlin brand (repeatability: 0.2% FS, long-term stability: < 1% of the measured value/year.

Compressed air: The maximum ventilation volume is designed according to 1.5~2 VVM, 500L ventilation volume: 0-1000L/min

The controller can realize: DO correlation cascade, curve analysis, batch report analysis, alarm, data storage, etc.

Exhaust system

With tail gas discharge condenser, the specially designed tail gas discharge pipeline has no liquid accumulation and no bacteria, and the exhaust condenser valve is manually controlled. The Swiss HUBA pressure sensor detects the tank pressure, and the German Proportional control valve controls the exhaust flow and the tank pressure.

Control range: 0-0.25Mpa.

Automatic sterilization

Use German Burket pneumatic valve; during sterilization, the controller automatically controls the opening and closing of the valve.

The temperature and holding time of each stage of sterilization can be set separately, equivalent sterilization or real-time sterilization can be switched, and the real-time curve display of the sterilization operation interface Sterilization temperature changes.

Tank sterilization temperature: 100~130°C, sterilization time: 0~60min, automatic sterilization when entering the filter, automatic sterilization of the tank (manual sterilization of the sampling valve), sterilization temperature error: ±0.5°C; The program is automatically controlled.

Stir system

Adopt Schneider servo motor + reducer and driver, mechanical stirring, special standard six-blade disc turbine and inclined blade for fermentation, the blade height is adjustable and different types of stirring blades can be replaced according to the special requirements of fermentation process Stepless speed regulation, 20L speed: 10-150rpm Stepless speed regulation, 200L speed: 10-150rpm The controller can realize: DO correlation cascade, curve analysis, batch report analysis, alarm, data storage, sequence control (set according to the fermentation time zone, automatic variable control, at least 10 segments) etc...

Temperature detection control

The jacket water bath is electrically heated, and the temperature control adopts PID automatic control mode, and the PID parameter adjustment can be realized on the control software. The Pt100 electrode can be sterilized repeatedly, and the electrode correction function can be completely corrected by the system software. Temperature control: cooling water temperature +5℃-65℃±0.2℃, display temperature: 0-150℃±0.1℃ Sequential control of the fermentation process (set according to the fermentation time zone, temperature automatic variable control, at least 10 stages)

PH control

Using intelligent PID control, high control accuracy, not easy to produce fluctuations, the original imported electrode and shielded wire detection from Switzerland, the peristaltic pump switch controls the flow of acid and alkali, automatic control, automatic measurement. The electrode can be sterilized repeatedly, and the calibration function is completely calibrated by the system software.

1. Swiss Mettler brand smart PH electrode (Inpro3253i/SG/120) and AK9 wire.

2. Swiss Mettler brand M300 dual-channel transmitter (M300 G2 Process 2-channel)

3. Display range: 0.00~14.00±0.01, automatic control range: 2.00~12.00±0.02 3.

4. Online detection, automatic alarm, peristaltic pump automatically add acid and alkali.

5. PID intelligent fuzzy control. The controller can realize: Ph value curve analysis, acid addition, alkali addition volume curve analysis, batch report analysis, acid addition volume cumulative display record, alkali addition volume cumulative display record, sequence control (set according to fermentation time section, automatic variable Control, at least 10 paragraphs) etc.

It is also equipped with Mettler I-link wire and Isense R2 software, which can refer to the use of a computer to analyze the electrodes, display the electrode status, the number of sterilizations, and generate electrode files for GMP verification.

DO control

Swiss Mettler optical electrode (Inpro6860i/120/mA) and VP8-ST shielded wire detection, DO value and speed, replenishment, air flow (if configured) and other parameters are associated and controlled.

Control range: 0~150%, ±3% fermentation process sequence control (according to fermentation time, DO automatic variable control, at least 10 stages).

It is also equipped with Mettler I-link wire and Isense R2 software, which can refer to the use of a computer to analyze the electrodes, display the electrode status, the number of sterilizations, and generate electrode files for GMP verification.

Feed control

Peristaltic pump switch control (manual, automatic, and off), automatic feeding and metering (according to fermentation time, automatic variable control of feeding, at least 10 stages).

With defoaming electrode, automatic detection of foam, peristaltic pump time ratio automatically add defoamer. The role of the peristaltic pump can be selected and set on the control interface, which makes the user more convenient and flexible in actual use. Equipped with specially designed feeding pins to ensure the safe operation of feeding.

Control system

1.  With industrial control computer combined with PLC control, it adopts standard display interface, which is controlled by external host computer. At the same time, it can be operated by keyboard and mouse. The sexualized screen is simple and clear, and the operation is simple and convenient. Control parameters: temperature, stirring speed, pH, dissolved oxygen, feed weight, defoaming, air flow, tank pressure, fermentation broth weight, exhaust O2, exhaust CO2, OUR, CER, RQ, KlaDisplay parameters: temperature, stirring speed, pH, dissolved oxygen, feeding, defoaming, air flow, pressure, automatic disinfection, (methyl alcohol, fermentation broth weighing, turbidity, exhaust O2, exhaust CO2, CER, OUR , Kla, RQ, bacterial volume, sugar, nitrogen, supplementary metering, potency and other parameters have been reserved for software functions, which can be upgraded later) In the future, if an exhaust gas analyzer is connected, the exhaust gas O2 and CO2 can be measured, and the offline parameters of CER, OUR, RQ, and KLa can be calculated online Note: The above are only software functions, which can only be realized after the user chooses to purchase the corresponding hardware.

2. The total interface can simultaneously display all the online parameter status of the seed tank and fermentation tank, such as: temperature, rpm, pH, dissolved oxygen, feed weight, defoaming, ventilation, pressure, etc.

3.  The software screen can display all parameter curves on the same interface, and can also set to display one or several parameter curves and set the range.

4.  The parameter curve can be shifted and enlarged to the time gradient before and after fermentation (the length of the time axis can be arbitrarily selected from 10 minutes to 20 days).

5.  Can choose online or historical parameter curve display and zoom in different time periods.

6.  One-click switching between real-time and historical curves, real-time curve restoration.

7.  Can set the "style", "color", "variable", "low limit" and "high limit" of the modified curve.

8.  The data analysis software can display the history of any batch fermentation process curve, and the display time range and vertical coordinate are flexibly adjustable. With offline data such as bacterial concentration, microscopic examination photos, amino nitrogen, viscosity and other parameters offline input and display curve capabilities, with the ability to export parameters to EXCEL table.

9.  Possesses flexible permission setting ability, distinguishing the operation permission of the staff.

10.  After the electrode calibration is completed, the system needs to display the slope and intercept of the linear equation to indirectly determine the electrode status (or similar indicators to determine the electrode status).